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Original Articles

Polyclonal Antibody Production Against Chito-Oligosaccharides

Pages 127-130 | Published online: 01 Jul 2010
 

Abstract

Chitin hexamer was conjugated to mono-BOC-diaminoethane, n -aminobutyric acid or glycine using reductive amination. Each hapten was conjugated to ovalbumin (OVA) or mouse serum albumin (MSA) for immunization and immunoassay, respectively. Serum from a mouse immunized with the diaminoethane conjugate, demonstrated competition with chitins in a modified indirect immunoassay.

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