Electrochemical Behaviour of Copper-Containing Nitrite Reductase from Alcaligenes Faecalis Strain-6

Yuzuru Iwasaki Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153, Japan

Makoto Nishiyama Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113, Japan

Sueharu Horinouchi Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113, Japan

Teruhiko Beppu Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113, Japan

Hideo Kadoi Saitama Institute of Technology, 1690 Ohaza Fuzaiji, Okabemachi, Ohosatogun, Saitama, 369-02, Japan

Shunichi Uchiyama Saitama Institute of Technology, 1690 Ohaza Fuzaiji, Okabemachi, Ohosatogun, Saitama, 369-02, Japan

Shuichi Suzuki Saitama Institute of Technology, 1690 Ohaza Fuzaiji, Okabemachi, Ohosatogun, Saitama, 369-02, Japan

Masayasu Suzuki Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153, Japan

Eiichi Tamiya Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153, Japan

Isao Karubem Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153, Japan

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Abstract

Indirect and direct electrochemical reactions of copper containing nitrite reductase (NIR) from Alcaligenes faecalis strain-6 are described. The reactivity of mediators, including blue protein from the same organism (the native redox partner of NIR, AfBP), in electrocatalytic reactions (EC') involving a mediator, NIR and nitrite was investigated. Several types of EC were observed and AfBP was found to be an effective mediator in spite of its high redox potential. Direct electrochemistry was observed at an Indium Tin Oxide electrode (ITO) and an edge plane oriented pyrolytic graphite electrode (PGE). Observation of the redox activity of NIR at an ITO in an optically transparent thin layer electrode cell (OTTLE) showed that it underwent reversible changes in absorbance that corresponded to the applied potential. The electrochemically adsorbed NIR at PGE showed fast electrochemical kinetics in cyclic voltammetry. It is suggested that the weak affinity of NIR to the PGE electrode may prevent complete denaturation of NIR in the adsorbed state.

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Electrochemical Behaviour of Copper-Containing Nitrite Reductase from Alcaligenes Faecalis Strain-6

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Electrochemical Behaviour of Copper-Containing Nitrite Reductase from Alcaligenes Faecalis Strain-6

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