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Journal of Liquid Chromatography Volume 6, 1983 - Issue 2
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Original Articles

Separation and Assignment of the Tryptic Peptides of Human Growth Hormone (hGH) and the 20K Dalton hGH Variant by Reversed Phase High Performance Liquid Chromatography

Milton T. W. Hearn St. Vincent's School of Medical Research Victoria Parade FITZROY, VICTORIA, 3065, AUSTRALIA
,
Boris Grego National Hormone Laboratory, University of Auckland Auckland, Department of Biochemistry, NEW ZEALAND
&
George E. Chapman National Hormone Laboratory, University of Auckland Auckland, Department of Biochemistry, NEW ZEALAND
Pages 215-228 | Published online: 05 Dec 2006
 
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Abstract

Reversed-phase high performance liquid chromatography (RP-HPLC) has been used to separate the peptides generated by tryptic cleavage of human growth hormone (hGH) and the 20K dalton human growth hormone variant. The total amino acid compositions of both these pituitary proteins has been accounted for on the basis of these chromatographic mapping procedures. Structural analyses of the peptides isolated from semi-preparative RP-HPLC separations has confirmed that the primary structure of the variant differs from that of the sequence of hGH by deletion of the amino acid residues 32–46.

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