Monoclonal antibodies specific for the organophosphate pesticide azinphos‐methyl

William T. Jones Plant Improvement Division, Horticulture and Food Research Institute of New Zealand, Batchelar Research Centre, Private Bag 11–030, Palmerston North, New Zealand

Dawn Harvey Plant Improvement Division, Horticulture and Food Research Institute of New Zealand, Batchelar Research Centre, Private Bag 11–030, Palmerston North, New Zealand

Stephen D. Jones Plant Improvement Division, Horticulture and Food Research Institute of New Zealand, Batchelar Research Centre, Private Bag 11–030, Palmerston North, New Zealand

Gordon B. Ryan Plant Improvement Division, Horticulture and Food Research Institute of New Zealand, Batchelar Research Centre, Private Bag 11–030, Palmerston North, New Zealand

Hans Wynberg Syncom, University of Groningen, Groningen, The Netherlands

Wolter Ten Hoeve Syncom, University of Groningen, Groningen, The Netherlands

Paul H. S. Reynolds Plant Improvement Division, Horticulture and Food Research Institute of New Zealand, Batchelar Research Centre, Private Bag 11–030, Palmerston North, New Zealand

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Abstract

2‐(2‐Mercapto‐5‐methyl‐1,3,2‐dioxaphosphorinan‐5‐yl,2‐sulphide)methoxyacetic acid has been synthesized and used to prepare an azinphos hapten and protein conjugates. Monoclonal antibodies of high affinity against the pesticide azinphos‐methyl were prepared from mice immunized with the hapten‐ovalbumin conjugate. The best monoclonal antibody could be used in an indirect enzyme‐linked immunosorbent assay (ELISA) to quantify azinphos‐methyl at concentrations from 50 pg ml^‐1 to 2 ng ml^‐1 (2.5–100 pg/assay). The corresponding limit of detection of the pesticide was less than 40 pg ml^‐1. Only the closely related pesticide azinphos‐ethyl showed any appreciable cross‐reactivity in this assay.

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Monoclonal antibodies specific for the organophosphate pesticide azinphos‐methyl

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